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Animals and Chemicals Male C57BL 6 mice Harlan Sprague Dawley, Hercules, CA ; were maintained with free access to food and tap water up to 1 before the experiment when food was removed. All studies were approved by the Animal Use Committee of the Greater Los Angeles Veterans Administration Healthcare System. DIDS, 5-nitro-2- 3-phenylpropylamino ; benzoic acid NPPB ; , methazolamide indomethacin, HEPES, and other chemicals were obtained from Sigma St. Louis, MO ; . PGE2 was obtained from Oxford Biochemical Oxford, MI ; . HEPES-saline solution contained 135 mM NaCl and 20 mM HEPES at pH 7.0. For.
From the Department of Biology, TechnionIsrael Institute of Technology, Haifa, Israel; and Department of Rheumatology, University Hospital, Vrije Universiteit VU ; , Amsterdam, The Netherlands. Submitted October 11, 2005; accepted November 15, 2005. Prepublished online as Blood First Edition Paper, December 20, 2005; DOI 10.1182 blood2005-10-4048. Supported by research grants of the Israel Cancer Association and the Star.
Provisions and other adjustments to shareholders' equity related to the merger Under French GAAP, the Group recorded adjustments to beginning shareholders' equity in the six months ended December 31, 1999 and each of the years ended December 31, 2000 and 2001 as a result of the finalization of estimates in conjunction with the merger of Sanofi and Synthelabo note D.12.2 ; , including provisions for restructuring, provisions for income taxes and other provisions. Depending on their nature, these French GAAP adjustments to shareholders' equity were eliminated under US GAAP and were recorded either i ; as an adjustment to goodwill recorded in connection with the merger or ii ; as adjustment to net income in the six months ended December 31, 1999 or one of the years ended December 31, 2000 or 2001. Sterling In September 1994, Sanofi acquired the worldwide assets of the human healthcare division of Eastman Kodak ``Sterling'' ; . Under French GAAP, no goodwill or intangibles associated with the acquisition of Sterling are reflected in the Sanofi~Synthelabo consolidated financial statements. Under US GAAP, certain intangible assets, including acquired in-process research and development, intellectual property rights and an assembled workforce, were valued, recorded and are being amortized over their estimated useful lives ranging from 8 to 20 years. The aggregate cost of Sterling to the Sanofi Group was approximately 940 million euro, excluding assumed liabilities. Other Under French GAAP, no goodwill or intangible assets associated with certain other acquisitions are reflected in the Sanofi~Synthelabo consolidated financial statements. Under US GAAP, certain intangible assets, including assembled workforce, were valued, recorded and are being amortized over their estimated useful lives. b ; Provisions and other liabilities.
ABSTRACT In this study, we have examined the potential of second-generation antisense chimeric 20 -O 2-methoxy ; ethyl DNA phosphorothioate oligonucleotides ONs ; to affect cell growth through non-antisense mechanisms. Evaluation of a series of ONs demonstrated that only a small number were cytotoxic at concentrations close to those required for antisense activity. Toxicity of the ONs appeared to be sequence dependent and could be affected by base and backbone modifications. Caspase-3 activation occurs with some ONs and it is most likely secondary to necrosis rather than apoptosis, since cells treated with toxic ONs did not show chromatin condensation, but did exhibit high-extracellular lactate dehydrogenase activity. Caspase-3 activation does not correlate with and appears not to be required for the inhibition of cell proliferation. Toxicity was only observed when ONs were delivered intracellularly. The mechanism by which one of the most cytotoxic ON produces cytotoxicity was investigated in more detail. Treatment with the cytotoxic ON caused disruption of lysosomes and Pepstatin A, a specific inhibitor of aspartic proteases, reduced the cytotoxicity of the ON. Reduction of lysosomal aspartic protease cathepsin D by prior treatment with cathepsin D-specific antisense ON did not attenuate the cytotoxicity, suggesting that other aspartic proteases play a crucial role in the cellular proliferation inhibition by ONs.
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Hornblow, Arthur. The end of the game. A novel. New York, G.W. Dillingham. c1907 Wright bibliography number 787; Ill. by A.E. Jameson. Reel: 84 Hornblow, Arthur. The lion and the mouse. A story of American life novelized from the play by Arthur Hornblow. New York, Grosset and Dunlap. c1906 Wright bibliography number 788; By Charles Klein; illustrated by Stuart Travis. Reel: 84 Hoskins, Bertha Ladd. The double fortune. New York, Neale Pub. Co. 1909 Wright bibliography number 789. Reel: 84 Hotchkiss, Chauncey Crafts. A prisoner of the sea. [New York], J. McBride. 1908 Wright bibliography number 790; Illustrated by Bert Knight. Reel: 84 Hough, Emerson. The way of a man. New York, Outing Pub. Co. 1907 Wright bibliography number 791; With ill. by George Wright. Reel: 84 Houk, L.C. Violett. The girl in question. A story of not so long ago. New York, J. Lane. 1908 Wright bibliography number 792. Reel: 84 Houston, Edwin James. At school in the cannibal islands. Philadelphia, Griffith and Rowland Press. 1909 Wright bibliography number 793. Reel: 84 Fuller, Henry B[lake]. Under the skylights. New York, D. Appleton and co. 1901 Wright bibliography number 831. Reel: 85 Fuller, Phoebe W. Shadows cast before. [a novel]. New York; London, The Abbey press. 1902 Wright bibliography number 832. Reel: 85 Fuller, Robert Higgison. The golden hope. A story of the time of King Alexander the Great. New York, The Macmillan company. 1905 Wright bibliography number 833. Reel: 85 Fuller, W[illiam] O[liver]. What happened to Wigglesworth. Boston, H.A. Dickerman & son. 1901 Wright bibliography number 834; Illustrated by E.D. Allen. Reel: 85 Fulton, Samuel. The Stoner family. [a novel]. New York; London, The Abbey Press. 1901 Wright bibliography number 836. Reel: 85 Gaines, Charles Kelsey. Gorgo. A romance of old Athens. Boston, Lothrop publishing company. [1903] Wright bibliography number 837. Reel: 85 Gallizier, Nathan. Castel del Monte. A romance of the fall of the Hohenstaufen dynasty in Italy. Boston, L.C. Page & company. 1905 Wright bibliography number 838; Illustrated by H.C. Edwards. Reel: 85 Hewes, Charles Edwin. The theatre terrible. A creation presenting various aspects of the greater drama. Chicago, Egerton-Palmer Press. 1910 Wright bibliography number 798. Reel: 85 Houston, Edwin James. The land of drought; or Across the great American desert. Philadelphia, Griffith & Rowland. c1910 Wright bibliography number 794. Reel: 85 Howard, Bronson Crocker. Kate. A comedy in four acts. New York, Harper & Brothers. 1906 Wright bibliography number 795; By Bronson Howard. Reel: 85 Howard, John Hamilton. In the shadow of the pines. A tale of tidewater Virginia. New York, Eaton and Mains. c1906 Wright bibliography number 796. Reel: 85 Howard, William Lee. Lila Sari. Boston, R.G. Badger. 1908 Wright bibliography number 797. Reel: 85!
Of atherosclerosis and also for the evaluation of preventive measures in randomized clinical trials. The ultrasound method is a promising technique, with a great potential for refined, computerized analyzing techniques. However, atherosclerotic manifestations are complex structures and, therefore, difficult to measure. Thus, there is a need for improved interaction between pathologists and researchers, who use different methods to measure atherosclerosis in vivo, to increase our understanding of what the recordings show. In addition, agreement about standardized measurement routines should be reached. This will simplify comparisons between different studies and will also aid uniform interpretation of results. Furthermore, more studies are needed to clarify the relation between the surrogate variables from large arteries e.g., carotid wall thickness and plaque ; and coronary heart disease. In doing so, we must be aware of the contrasting goals for the clinician and the epidemiologist and the fact that clinical patient groups are not representative of the population at large. Hard end points like myocardial infarction and sudden death are usually due to a combination of an underlying atherosclerotic disease and triggering or precipitating factors such as left ventricular electric instability, plaque instability, or a disturbed thrombogenesis-thrombolysis balance. It might be advisable, therefore, to combine different surrogate variables for both the underlying atherosclerotic disease and triggering or precipitating mechanisms ; to obtain a broader perspective of the disease process and methenamine.
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Both sunitinib and temsirolimus are considerably more active than IFN as first-line treatment for patients with mRCC Sunitinib is indeed a new reference standard for first-line RCC Rx Temsirolimus is the first new agent to significantly prolong OS The addition of IFN to temsirolimus did not improve efficacy in this poor prognosis patient-population. In fact, lowering the dose of temsirolimus in order to accommodate IFN side effects may have compromised clinical benefit. Both agents appear well tolerated relative to IFN Side effect profiles of temsirolimus and sunitinib appear different, suggesting they may have at least partially nonoverlapping mechanisms of action and methimazole
Academia is still the place where innovation starts. But the translation from a research concept to a market product is a lengthy and risky process. Biotechnology spin-outs, like all innovation based start-ups, need different funding options. In today's financial environment, pre-seed and seed funding appear more and more essential in order to fill the primary equity gap and to prepare start-ups to the next funding round. The objective of this session is to provide an overview of the numerous seed capital fund models that have emerged, and to discuss issues like fund strategy, management skills, risk profiling, exits, and the bargaining power of seed capital funds. Coordinator: Jean-Pierre Loza, Head of Department, INSERM-Transfert Stphane Mry, Fund Manager, Bloomsbury Seed Fund, UK Conny Bogentoft, Managing Director, Karolinska Innovations, Sweden Janis Anderson, Chairwoman, Javelin Ventures, London Bioscience Innovation Centre Stephen Herr, Managing Director, EMBL Ventures GmbH - Germany.
Updated information and services can be found at: : bloodjournal.hematologylibrary cgi content full 105 5 2066 Articles on similar topics may be found in the following Blood collections: Signal Transduction 1920 articles ; Immunotherapy 571 articles ; Immunobiology 3408 articles ; Neoplasia 3910 articles ; Information about reproducing this article in parts or in its entirety may be found online at: : bloodjournal.hematologylibrary misc rights.dtl#repub requests Information about ordering reprints may be found online at: : bloodjournal.hematologylibrary misc rights.dtl#reprints Information about subscriptions and ASH membership may be found online at: : bloodjournal.hematologylibrary subscriptions index.dtl and methocarbamol.
P-gp in normal tissues of the body seems to corroborate this hypothesis. P-gp is found on the apical surface of intestinal and renal epithelia and the secretory glands of the endometrium in pregnant mice Croop et al., 1989 ; . In the brain, P-gp is expressed on the subapical side of the choroid plexus epithelia Rao et al., 1999 ; and on the luminal surface of brain microvessels Beaulieu et al., 1997 ; . Recently, Golden and Pardridge 1999 ; observed the expression of P-gp on the abluminal side of the blood-brain barrier on neighboring astrocyte foot-processes. At present, the functional expression of P-gp in microglia, the primary target and reservoir of HIV-1 in the brain, is unknown. The goal of this project was to investigate the functional expression of P-gp in brain parenchyma, by using a rat brain microglia cell line MLS-9 ; . This cell line has been well characterized and when grown to confluence exhibits several morphological and functional properties of activated microglia seen in vivo. That is, MLS-9 cells round up like phagocytic microglia, they express high levels of complement C3 receptors and lysosomes, and can produce large amounts of nitric oxide Schlichter et al., 1996; Zhou et al., 1998.
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Consider revaccination 3 years after previous dose. Evidence of effectiveness A meta-analysis by Cornus et al 2 based on 14 randomised trials confirms unambiguously the high efficacy of pneumococcal polysaccharide vaccine in reducing definite bacteremic ; pneumococcal pneumonia by 71% and presumptive pneumococcal pneumonia by 40% with a possible 32% reduction in mortality due to pneumonia. Level 1.
FIG. 4 The effects of HCOi-free medium, inhibition of car. bonic anhydrase, and SITS on carbachol-induced acidification. BCECF-loaded acini were suspended in PSS trace A ; , HCOT-free PSS trace E ; , PSS plus 1 m methazolamide trace C ; M or PSS plus 1 m SITS trace D f , all containing 0.5 m amiloride. M M M carbachol was added to At the times indicated by the arrows, the cuvette. Initial ratesof acidification after carbachol addition were 0.53 f 0.04units min trace A, n 9 ; , 0.19 f 0.02 units min trace B , n 4 ; , 0.22 f 0.01 units min trace C, n 3 ; , and 0.51 f 0.08 trace D, n 4 and methylcellulose.
Two treatment groups LD 93 patients, 33.1%; CD 96 patients, 37.2% ; , and there were no significant differences between doses for either gender female P 0.616; males P 0.347 ; or either onset CO P 0.900; AO P 0.274 ; . Overall from baseline to 6 months, fewer patients in the lower-dose group reported at least one adverse event LD 169 patients, 56.0%; CD 194 patients, 66.2%; P 0.010 ; . Between-dose differences were not significant for females P 0.056 ; , males P 0.060 ; , or CO patients P 0.423 ; but were significant for AO patients P 0.008 ; . The distribution of GH-related adverse events by treatment group during the study is shown in Table 4. Arthralgia was the only event that occurred significantly more frequently in the CD group.
22. Constant, M. A., and Becker, B.: The effect of carbonic anhydrase inhibitors on urinary excretion of citrate by humans, Am. J. Ophthalmol. 49: 929, 1960. Stone, R., Shin, D. S., Zimmerman, T., et al.: Low-dose methazolamide and intraocular pressure, Arch. Ophthalmol. in press ; . 24. Maren, T. H.: Renal carbonic anhydrase and the pharmacology of sulfonamide inhibitors. In Herken, H., editor: Handbook of Experimental Pharmacology, Berlin, 1969, SpringerVerlag, vol. 24, p. 195. 25. Wistrand, P. J., and Maren, T. H.: The effect of carbonic anhydrase inhibition on intraocular pressure of rabbits with different blood CO2 equilibria, Am. J. Ophthalmol. 50: 291, 1960. Bietti, G., Virno, M., and Pecori-Giraldi, J.: Acetazolamide, metabolic acidosis, and intraocular pressure, Am. J. Ophthalmol. 80: 360, 1975. Becker, B.: Carbonic anhydrase and the formation of aqueous humor Friedenwald Memorial Lecture ; , Am. J. Ophthalmol. 47: 342, 1959. Maren, T. H.: HCO3~ formation in aqueous humor: mechanism and relation to the treatment of glaucoma, INVEST. OPHTHALMOL. 13 and methyldopa.
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INTRODUCTION The most important aim of cancer therapy is to increase the survival time of cancer patients, enabling them to live in comfort. Anticancer chemotherapy, however, is generally accompanied by severe toxic side effects and reduces the host's resistance to cancer and infectious diseases, especially destroying lymphoid cells and bone marrow cells. Therefore, potentiation of host resistance should be one of the most important objectives in the development of cancer and AIDS therapy 5 . Recently, immunochemotherapy has been attempted and methysergide.
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C.Savon1, A.Goyenechea 1, O.Valdes1, G.Gonzalez1, L. Palerm1, G. Gonzalez-Baez1, P. Perez-Brena 2, J. Barrios 1. 1"Pedro Kouri" Institute, Havana City, Cuba; 2Instituto de Salud Carlos III, Madrid, Spain Background: Respiratory Syncytial Virus RSV ; is leading cause of serious lower respiratory tract infections infants. Comorbid conditions such as chronic diseases, prematurity have been associated with greater disease severity illness, but information about the relationship of RSV genotypes and severity illness is still lacking. Methods: Forty selected strains of RSV group A and isolated B from Cuban infants with acute respiratory disease over five seasons were studied.Viral RNA was extracted and polymerase chain reaction PCR ; was carried out using primers directed to parts of the N and F gene respectively. Amplicons were digested using restriction fragment-length polimorphism RFLP ; to define the association between virus and severity disease. Disease severity was assessed as very mild, mild, moderate and severe. Results: Three of the six known N genotypes were detected, but NP4 and NP3 were found most frequently, moreover was difficult to establish a relationship between N genotype and disease severity. Five F genotypes were found F1, F2, F5, F9 and F11; besides F9 and F11 were associated with very mild disease severity, although F1 genotype appears to be related with moderate to severe disease and metolazone.
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