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1. Stevens JM, Kurtz A. Eckardt KU, Winearls CG. Anaemia in chronic renal failure. In: S Cameron, Davison, JP Griinfeld, D Kerr, E Ritz, eds. Oxford Textbook of Clinical Nephrology. Medical Publications, Oxford, 1992; 1344-1360 2. Winearls CG, Oliver DO, Pippard MJ, Reid C, Downing MR, Cotes PM. Effect of human erythropoietin derived from recombinant DNA on the anaemia of patients maintained by chronic haemodialysis. Lancet 1986; 2: 1175-1178 Eschbach JW, Egrie JC, Downing MR, Browne JK, Adamson JW. Correction of the anemia of end-stage renal disease with recombinant human erythropoietin. Results of a combined phase I and II clinical trial. N Engl J Med 1987; 316: 73-78 Besarab A, Caro J, Jarrell BE, Francos G, Erslev AJ. Dynamics of erythropoiesis following renal transplantation. Kidney Int 1987; 32: 526-536 Goch J, Birgegard G, Wikstrom B, Tufveson G, Danielson BG. Serum erythropoietin levels in the immediate kidneyposttransplant period. Nephron 1992; 60: 30-34 Carson JL, Willett LR. Is a Hemoglobin of 10 g required for surgery ? Med Clin North 1993; 77: 335-347 Nelson AH, Fleisher LA. Rosenbaum SH. Relationship between postoperative anemia and cardiac morbidity in high-risk vascular patients in the intensive care unit. Crit Care Med 1993; 21: 860-866 Stevens JM, Strong CA, Oliver DO, Winearls CG, Cotes PM. Subcutaneous erythropoietin and peritoneal dialysis. Lancet 1989; 1: 1388-1389 letter ; 9. Jindal KK, Hirsch DJ, Belitsky P. Whalen MA. Low-dose subcutaneous erythropoietin corrects the anaemia of renal transplant failure. Nephrol Dial Transplant 1992; 7: 143-146 Kessler M, Legrand E, Mertes M, Renoult E, Hestin D. Treatment of chronic renal failure anemia by recombinant erythropoietin and polycythemia following kidney transplantation. Nephron 1992; 62: 370-371 Traindl O, Barnas U, Franz M et al. Recominbant human erythropoietin in renal transplant recipients with renal anemia. Clin Transplant 1994; 8: 45-48.
Tamed for several 11Ccompounds studied in our laboratory Table 2 ; . The rationale is that in each time interval the.
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FIG. 5. HOXA9 and MN1-TEL cooperatively cause acute myeloid leukemia AML ; in mice. A. Expression of HOXA9 in AML patient samples with MN1-TEL. A quantitative RT-PCR result is.
Next we assessed the frequency of Treg cells in context of stage of disease in previously untreated patients with CLL Figure 4 ; . Of all 73 patients, 26 were previously untreated. This analysis revealed a correlation between Treg cell frequency and stage of disease with highest numbers of Treg cells in patients with extended disease Binet C ; followed by patients with Binet B and with lowest but still increased numbers in Binet A. In patients in Binet stage B and especially in Binet stage C we also observed an increase of CD4 CD25hi T cells coexpressing GITR, CD62L, and intracellular CTLA4 compared to healthy controls data not shown.
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Mechanical obstruction of pulmonary capillaries with white cells was postulated by these investigators to be the most likely explanation for the pulmonary dysfunction observed following the onset of hemodialysis in patients, and for the rise in pulmonary artery pressure observed in sheep following infusion of autologous complement-activated plasma Craddock et al., 1977a, 1977b, 1978 ; . We previously have observed a similar pulmonary response in sheep after the onset of venovenous membrane oxygenator perfusion using a silicone rubber oxygenator Birek et al., 1976 ; . However, our finding that pretreatment of the animal with sulfinpyrazone abolished the pulmonary response without altering the leukopenic response cast doubt that leukostasis alone was an adequate explanation for the pulmonary response following complement activation. The present study was designed to investigate further the relationship between complement-mediated leukopenia and alterations in pulmonary vascular resistance and pulmonary gas exchange and sulindac.
Abstract Background. The percentage of hypochromic red blood cells RBCs ; %HYPO ; has been demonstrated as the best predictor of response to iron loading in haemodialysis patients treated with recombinant human erythropoietin rHuEPO ; . However, we have previously shown that this parameter is positively influenced by erythropoietic activity since reticulocytes are considered hypochromic by cell counters. New cell counters are able to determine cell volume and haemoglobin Hb ; concentration separately on reticulocytes and mature erythrocytes. The aim of this study was to assess the sensitivity and specificity of mature erythrocyte parameters in detecting functional iron deficiency FID ; . Methods. A total of 32 stable chronic haemodialysis patients in the maintenance phase of rHuEPO therapy were included. Classical parameters of iron monitoring and mature erythrocyte parameters were measured after a 4-week iron-free period. Patients were classified as responders R ; or non-responders NR ; to an iron load of 100 mg iron sucrose at each dialysis session for 4 weeks, according to whether their Hb increased by 1 g the end of iron loading. Results. Twelve patients were identified as responders. Receiver operating characteristic ROC ; curve analysis demonstrated %HYPO and its corresponding parameter on mature erythrocyte, %HYPOm, as the best predictors of FID. The other parameters were ordered as follows: tranferrin saturation TSAT ; , ferritin FRT ; , mature RBC Hb content CHm ; , mean corpuscular Hb concentration MCHC ; , percentage of mature erythrocytes with a low CHm %lowCHm ; , mean content in Hb MCH ; and reticulocyte Hb content CHr. Comparing the parameters at different cut-offs, the best sensitivity, specificity and efficiency were demonstrated for %HYPOm 6.
Accordance with the for the Care and Use of Laboratory Animals. Preparation of epididymal adipocytes Epididymal adipocytes were isolated by collagenase digestion of the epididymal fat pads of male Sprague-dawley rats 59 ; and after being washed free of enzyme, were suspended 1: 5 V Krebs-Ringer phosphate buffer containing 1% bovine serum albumin BSA ; and 5 mM glucose. Aliquots of cell suspension 600ml ; were incubated without or with each nucleotide for lipolysis and incubated in a quartz cuvette with 10 M Fura2 AM at 37 for 40 min under continuous stirring for calcium measurement. Western blot analysis Adipocytes were plated in 60-mm tissue culture dishes and treated with nucleotides for various times as indicated. After treatment, the cells were washed twice with cold phosphate-buffered saline and then lysed with lysis buffer 250 mM Tris-Cl pH 6.5 ; , 2 % SDS, 4 % -mercaptoethanol, 0.02 % bromophenol blue, and 10 % glycerol ; . Equivalent amount of proteins were resolved by SDS-PAGE and analyzed by Western blotting. Immunoblot analysis was performed as we previously described 57 ; . Signals were detected with an ECL detection system Neuronex Co., Korea ; . Determination of intracellular Ca2 + level The level of intracellular Ca2 + was measured using the fluorescence Ca2 + indicator fura-2 AM1 as previously described 23 ; . Briefly, after the differentiated adipocytes were harvested from 100 mm culture plates by 0.25% w v ; trypsinization, the cells were washed with serumfree M199 medium. Then cell suspensions were incubated in a quartz cuvette with 3 M fura2 AM at 37 for 40 min under continuous stirring. The cells were then resuspended in Locke's solution 154 mM NaCl, 5.6 mM KCl, 2.2 mM CaCl2, 1.2 mM MgCl2, 10 mM glucose, and 5 mM HEPES, pH 7.4 ; . In Ca2 + -free Locke's solution, CaCl2 was omitted and 100 M EGTA was included. Sulfinpyrazone 250 M ; was added to all solutions to prevent dye leakage 24 ; . Changes in fluorescence ratio were measured at the dual excitation wavelengths of 340 nm and 380 nm and the emission wavelength of 500 nm. [Ca2 + ]i was calculated using the equation: [Ca2 + ]i and surmontil.
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Technical environments including the use of ITIL in multi-sourced IT environments. Other goals include improving the usefulness and applicability of ITIL by addressing the changing needs of users as the technology base and business requirements continue to evolve, and making ITIL easier to apply and improve its applicability to small organizations. The Core Components The Core is a new set of 5 books that follow a lifecycle model from design to retirement. The Core is to include the key concepts and generic best practices that do not change rapidly. Much of the Service Support and Service Delivery remains, but is split out among the 5 books of the Core in a more cyclic and business oriented framework. The working titles are.
Concentration corresponding to the apparent Km or S50 value of each enzyme Sorich et al., 2002; Uchaipichat et al., 2004 ; . Concentrations of amitriptyline, phenylbutazone, quinidine, quinine and sulfinpyrazone used in screening experiments were 0, 10, 100, 500 and 1000 M. The concentrations of hecogenin used for inhibition screening were 0, 1, 10, 100 and 200 M, while concentrations of androsterone and canrenoic acid were 0, 1, 10, 100 and 500 M. Negative controls for the 4MU assay contained each putative inhibitor and substrate i.e., 4MU ; without UDPGA whereas negative controls for TFP assay contained inhibitor and UDPGA without substrate ; . Amitriptyline and quinidine available as salts ; were dissolved in water but androsterone, canrenoic acid, phenylbutazone, quinine, sulfinpyrazone and hecogenin required addition of an organic solvent, which was selected on the basis of known effect on enzyme activity Uchaipichat et al., 2004 ; . Up to 2% methanol v v ; was selected for incubations with all UGTs except UGT 1A6 and 2B15. Incubations with these enzymes contained 1% methanol v v ; for the dissolution of androsterone, canrenoic acid, phenylbutazone, quinine and hecogenin, while 1% DMSO v v ; was used for incubations containing sulfinpyrazone. Control incubations contained the same concentration of organic solvent. Subsequent experiments that determined inhibitor constants Ki ; for selected compounds included 4 inhibitor concentrations at each of three substrate concentrations. The Ki values of phenylbutazone and quinine were investigated for those enzymes exhibiting Michaelis-Menten 4MU glucuronidation kinetics viz. UGT 1A1, 1A6, 1A7 and 1A10 ; and for UGT1A9 `weak' substrate inhibition ; . In addition, AZT was used as the substrate for the determination of the Ki value for quinidine and symlin.
Remicade or infliximab is a monoclonal antibody salsalate is used to relieve mild pain, to reduce fever, and to reduce the pain and inflammation swelling ; caused by arthritis or other inflammatory conditions sulfasalazine or azulfidine is used to prevent and treat inflammatory bowel disease and rheumatoid arthritis sulfinpyrazone or anturane is used to treat gouty arthritis.
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[ ] Temple University Philadelphia, Pennsylvania EdM--Masters, Marketing & Education, College of Education Masters degree will be completed by 12 06 Temple University Philadelphia, Pennsylvania, 12 93 BA, School of Communications and Theater, Radio-Television-Film Temple University London, England School of Communications and Theater, 1992 [things i've done] CONSULTING Adam Bailine Freelance Advertising Marketing Consulting 1 96present Creative Director Copywriter Consultant. Develop and write copy concepts for clients in industries like: pharmaceutical, financial, retail, healthcare, utilities, and other consumer and B2B companies. Work on Web, print, multimedia, broadcast, direct response, PR, corporate ID, branding, marketing communications, and music projects. Produce & direct broadcast video projects. Through my consulting I worked for the following companies: Roska Direct Direct Response Agency 5 012 02 Creative Director Consultant ; . Worked on-site at Roska Direct on pharmaceutical and consumer accounts. Wrote copy concepts and managed art directors, designers, copywriters, and information architects on multimedia, print, PR, and direct response advertising and marketing projects. Interfaced with clients, pitched new business, and gave insight into production schedules and budgets. iSun Networks Internet Technology Company 6 002 01 Senior Creative Director Consultant ; . Built and managed iSun's in-house agency. Played a large role in all branding marketing PR efforts for iSun's consumer & B2B products. Guided the creative & production process, from concept to completion. Wrote and designed copy and concepts for Traditional and Interactive projects, including Web, collateral, ads, broadcast, multimedia, and direct response. Led 15 Web & print designers, illustrators, copywriters, Web content producers, information architects, BeyondMedia Interactive & Traditional Agency 12 994 00 Senior Creative Director Consultant ; . Built, led, and organized the creative, production, and account teams. Managed the creative & production process, from concept to completion. Wrote and designed copy and concepts. Headed up a staff of 14, including marketing managers, art directors, Web & print designers, copywriters, programmers, information architects, and production. Managed client services staff, client contact, and outside vendors. Headed up PR, self-promotion, and corporate ID projects, including a Web site designed specifically to bring in new business and symmetrel.
Purified recombinant poly hydroxyalkanoic acid ; , PHA, synthase from Chromatium vinosum was used to examine in vitro poly 3-hydroxybutyric acid ; P 3HB formation. In combination with purified propionylcoenzyme A transferase of Clostridium propionicum As a direcxt method for the evalualtion of tissue bonding to two polyhydroxybutyrate PHB ; based composites, a mechanical push-out test was perfomred on implants inthe femur of mature Japanesse White rabbits. Three composites were tested. The first, a hyd Application natural abundance solution 13C nuclear magnetic resonance n.m.r. ; spectroscopy was used to monitor the intracellular production and degradation in vivo of two poly 3hydroxyalkanoates ; PHAs ; , produced by Pseudomonas Oleovorans , poly 3hydroxynonanoate.
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Once again this year Quirino's definition of the province of Ragusa seems to perfectly describe an entity which take on the form of "An island within an island". This province represents the best example of how the growth of the southern region of Italy can derive not from the acritical reproposition of the industrial development course travelled by the North of Italy during the 1960s and 70s, but via the intelligent enhancement of its specificities. Ragusa has been able to reconcile a high growth rate in economic activities and a decrease in unemployment with the production-related specialization which, at one time, would have been considered as a sign of underdevelopment: specialization in the agricultural field. We should add, however, that Ragusa's agriculture is specialised in the sector characterised by the highest level of value added horticulture ; and that, to date, it is heavily conditioned by the lack of adequate transport infrastructures. The year 2000 also saw growth in a series of manufacturing food and textiles ; and service sectors mainly financial and property ; bearing witness to the fact that it is possible to adapt the needs of the various sectors traditional and advanced ; even within a limited territory such as the provincial area and synagis
1. Kent DM, Price LL, Ringleb P, Hill MD, Selker HP. Sex-based differences in response to recombinant tissue plasminogen activator in acute ischemic stroke: a pooled analysis of randomized clinical trials. Stroke. 2005; 36: 62 Schulz KF, Grimes DA. Multiplicity in randomised trials II: subgroup and interim analyses. Lancet. 2005; 365: 16571661. Collins R, MacMahon S. Reliable assessment of the effects of treatment on mortality and major morbidity, I: clinical trials. Lancet. 2001; 357: 373380. Canadian Co-operative Study Group. A randomised trial of aspirin and sulfinpyrazone in threatened stroke. N Engl J Med. 1978; 299: 5359. Antiplatelet Therapy Trialists' Collaboration. Collaborative overview of randomised trials of antiplatelet therapyI: Prevention of death, myocardial infarction, and stroke by prolonged antiplatelet therapy in various categories of patients. Antiplatelet Trialists' Collaboration. [see comments]. [erratum appears in BMJ 1994 Jun 11; 308 6943 ; : 1540]. BMJ. 1994; 308: 81106. KEY WORDS: XXXXX.
Probenecid, sulfinpyrazone uricosuric effect of these agents may be decreased by large doses of aspirin, in addition, renal excretion of salicylate may be reduced and synvisc.
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The history of the development of pharmacy has been well covered elsewhere Hepler 1987; Lubbe 2000; Traulsen & Bissell 2004 ; . What follows provides a brief overview of that history Table 6-i ; , and some reflection on the impact of aspects of the history on pharmacy practice and tace
ECAUSE of the narrow therapeutic window, cytotoxic chemotherapy is often associated with considerable toxicity. Attempts therefore have been made to develop drugs with novel actions with minimal side effects. 8-chlorocAMP 8-Cl-cAMP ; is one of a new generation of drugs that act at the level of signal transduction to modulate the activity of cAMP-dependent protein kinase PKA ; . PKA is tetrameric having two catalytic and two regulatory subunits. There are two main isoenzymes PKAI and PKAII ; , each sharing a common catalytic unit but differing by virtue of distinct regulatory subunits, termed RI and RII. Both regulatory subunits have and forms, which are present in varying proportions in different tissues. Two molecules of cAMP are able to bind to each regulatory subunit, one at each of two separate regions, termed site 1 and site 2 1, 2 ; . Enhanced expression of PKAI or RI- ; has been associated with cellular proliferation, whereas up-regulation of PKAII or RII ; has been correlated with growth inhibition and cellular differentiation. In vitro studies in tumor cell lines have shown that an increase in the RI RII ratio can stimulate rapid uncontrolled growth 2, 3 ; . Overexpression of R1- has also been shown to be an indicator of a poor prognosis in patients with breast cancer 4, 5 ; . The ratio of RI to RII is therefore an!
| Serum-free RPMI 1640 medium with 3 M fura-2 AM at 37C for 40 min. Sulfinpyrazone 250 M ; was added to all solutions to prevent dye leakage. Changes in fluorescence ratios were measured at the dual-excitation wavelengths of 340 and 380 nm and the emission wavelength of 500 nm. [Ca2 ]i was calculated using the equation [Ca2 ]i Kd[ R Rmin ; Rmax R ; ] Sf2 Sb2 ; , where Rmax and Rmin are the ratio obtained when fura-2 is saturated with Ca2 and when EGTA is used to remove Ca2 , respectively. Sf2 and Sb2 are the proportionality coefficients of Ca2 -free fura-2 and saturated fura-2, respectively. Calibration of the fluorescence signal in term of [Ca2 ]i was performed according to Grynkiewicz et al. [15]. In extracellular Ca2 -free experiments, the Locke's solution did not contain Ca2 ion but included 200 M EGTA and tacrine.
Unlike probenecid, the effect of sulfinpyrazone on the plasma levels of weak organic acids such as penicillins does not appear to be clinically relevant and sulindac
A very large number of drug interactions have been reported with warfarin. The drugs may interact through pharmacodynamic or pharmacokinetic mechanisms. The former include alteration of bioavailability of vitamin K, affecting receptor sensitivity and affecting haemostasis via platelet function. The interactions of a pharmacodynamic nature occurring with one anticoagulant may well apply to another, while this is not necessarily the case with interactions of a pharmacokinetic nature. The pharmacokinetic interactions may be due to effects on warfarin absorption, protein binding and metabolism. Few drugs have been shown to alter warfarin absorption, the importance of protein binding displacement has been exaggerated, and since warfarin is eliminated in a very little extent unchanged by kidney the most important kinetic interactions are those due to inhibition or induction of its metabolism. Examples of hepatic microsomal enzyme-inducing drugs are barbiturates, griseofulvin, rifampicin, phenazone and aminoglutethimide. Inhibition of metabolism of warfarin has been demonstrated with many drugs, such as H2-receptor antagonists and proton pump inhibitors, phenylbutazone, metronidazole, co-trimoxazole, amiodarone, sulfinpyrazone and macrolide antibiotics. Some drugs may interact by more than one mechanism. In several cases both increased and decreased anticoagulation have been reported for the same drug, so some interacting drugs do not produce predictable effect. The clinically significant interactions have been reported with drugs containing medicinal herbs, such as feverfew, garlic, ginger, ginkgo, ginseng, St. John's Wort, mountain arnica, camomile, fenugreek, primrose and common celery. Foods or nutritional containing vitamin K, avocado pears, ice cream, grapefruit juice and ethanol may affect the anticoagulant effect of warfarin. Because of possible serious consequences of interference with anticoagulant therapy, special care is required when any medication is added to or withdrawn from patients on anticoagulant therapy. Patient monitoring should be more frequent in such cases. Caution is and tamiflu.
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