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Spectinomycin resistance in clinical isolates of Neisseria meningitidis and Neisseria gonorrhoeae was found to be due to mutations G1064C and C1192U Escherichia coli numbering ; in 16S rRNA genes, respectively. Among Neisseria species, only N. meningitidis and N. gonorrhoeae are considered primary pathogens 8 ; . Strains of N. gonorrhoeae are always pathogenic, whereas strains of N. meningitidis, in addition to causing acute meningitis and septicemia, can also colonize the oro- and nasopharynx of a healthy carrier. Development of drug resistance in N. gonorrhoeae has led to concern about the almost inevitable increase of resistance in N. meningitidis 8 ; . Cephalosporins and fluoroquinolones are the two classes of antibiotics recommended for primary therapy 8 ; . Resistance to alternative therapy such as chloramphenicol for meningococcal infections 5 ; or spectinomycin for gonococcal infections 1 ; has been reported. In gram-positive bacteria, resistance to spectinomycin, although much less common than resistance to other aminoglycoside-aminocyclitol antibiotics, is usually due to production of an aminoglycoside 9-O-nucleotidyltransferase of type I 9, 12 ; . Recently, a spectinomycin phosphotransferase has been reported for the gram-negative Legionella pneumophila 16 ; . In Escherichia coli, spectinomycin resistance has been shown to be due to mutations in helix 34 of 16S rRNA 15 ; . This helix consists of an upper and a lower stem separated by an internal loop containing two uracil residues. We report mutations responsible for spectinomycin resistance in 16S rRNA genes of clinical isolates of N. meningitidis and N. gonorrhoeae. Since 1988, surveillance of antibiotic resistance in clinical isolates of Neisseria spp. has been conducted at the National Center for Meningococci, Institut Pasteur, Paris, France, by disk-agar diffusion. Out of more than 16, 800 clinical isolates, a single N. meningitidis isolate, LNP16311, and four N. gonorrhoeae isolates were found to be resistant to spectinomycin. The five strains of Neisseria spp. remained susceptible to penicillins, cephalosporins, tetracyclines, macrolides, rifamycins, and quinolones. Among the three N. gonorrhoeae strains isolated from urethritis patients in Gabon LNP8205, Libreville, May 1989; LNP8919 and LNP8920, Franceville, March 1990 ; , only strain LNP8205, as well as strain LNP9455, isolated from a urethritis patient in November 1990 at Saint Louis Hospital in Paris, was studied further. N. meningitidis LNP16311, serogroup Y, was isolated in 1998 from the rhinopharynx of a 71-year-old male in Macon, France. A spectinomycin-resistant transformant, N. meningitidis BM4417, obtained after transformation of strain BM4377 5, 13 ; with total DNA from LNP16311, was included in the study. Spectinomycin-susceptible N. meningitidis LNP15908.

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The record recruitment of new United Spinal members is archived in our membership database, and maintained and updated by dedicated staff. We have developed educational booklets and pamphlets, news magazines, presentations, and other media that helped to draw new members, donors, and corporate partners to our association. United Spinal's sponsorship of annual continuing education conferences for health professionals in the spinal cord disability field required thousands of hours of staff preparation of conference materials, meeting planning, and arrangement of travel and hotel accommodations throughout the year. Without our hard-working staff, the above achievements and others you will soon read about would simply not occur. It took every United Spinal employee to realize the growth that we enjoyed in FY 2005.

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Division of Pharmacology, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands 2 Takeda Chem . Industries Ltd., 17-85, Jusohonmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan 3 LAP&P Consultants BV, Archimedesweg 31, 2333 CM Leiden, The Netherlands 4 Epilepsy Institute of The Netherlands, SEIN, P.O. Box 21, 2100 AA Heemstede, The Netherlands PK PD -experiments with subunit selective GABAergic ligands were performed before and after SE. The data were analysed using the model reported by Visser et al1. It is hypothesized that changes in functionality of the GABAA -receptor are caused by alterations in subunit composition. Therefore, only changes in the in vivo intrinsic efficacy ePD ; were assumed Tetracycline 43 83 * a Chloramphenicol 0 5 1 Ampicillin -b 15 41 * 51 * Cephalothin 3 7 2 Sulfonamide 15 45 * 47 * Erythromycin 34 33 0 Penicillin 1 0 Trimethoprim 3 30 * 42 * Spectinomycin 1 15 * 7 Streptomycin 8 18 7 Naldixic aid 6 24 * 18 * Ciprofloxacin 0 4 9 Gentamycin 0 5 0 Neomycin 0 3 2 No. of isolates 74 40 216 a * Significant higher prevalence of resistant isolates when compared to Danish broiler meat 95% confidence interval ; . b-; not determined. One Ceftiofur resistant E. coli from a poussin is not included in the table. 118.

We welcome you to this celebration of worship. Newcomers and members are invited to give the requested information on the Worship Registration Pad as it is passed along the pew during the Children's Message. Name Tags are in the pews for everyone to use. Please join us after worship for fellowship and coffee in the Parish Hall and spiriva.

Management of pregnant APL patients Management of APL during pregnancy is always a cause of major concern due to the hemorrhagic risk and the potential teratogenicity of ATRA and chemotherapy. However, in contrast to the experience reported in the pre-ATRA era, 40 all cases described so far in which ATRA was used alone or in combination with chemotherapy attained CR and no serious adverse effect were recorded for either the mother or the fetus.41 The limited experience available from the literature suggests that both ATRA and anthracycline-based chemotherapy appear reasonably safe for APL patients diagnosed in the second or third trimester of pregnancy, as they do not seem to compromise the delivery of a healthy newborn. In fact, the products of all the pregnancies reported, although premature, survived and developed normally. Nevertheless, close fetal cardiac monitoring to unravel complications has been strongly recommended throughout the pregnancy because some. To cause a release of very-high-molecular-weight VWF multimers independently of rhIL-11 treatment Figure 5 ; . This was especially pronounced on day 6, the first day of DDAVP treatment compare Figure 5; day 6, before treatment, and day 6, 30 minutes ; . As expected, the rhIL-11 treatment produced an increase in platelet counts by 66% 282 66 to 469 112 106 mL by day 12 ; , and a 154% increase in total fibrinogen 223 9 to 566 110 mg dL by day 7 ; complete data not shown and ssd.

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The morphology of Arabic poses special challenges to computational natural language processing systems. The exceptional degree of ambiguity in the writing system, the rich morphology, and the highly complex word formation process of roots and patterns all contribute to making computational approaches to Arabic very challenging. Indeed many computational linguists across the world have taken up this challenge over time, and many of the researchers with a track record in this research area have contributed to this book. The book's subtitle aims to reflect that widely different computational approaches to the Arabic morphological system have been proposed. These accounts fall into two main paradigms: the knowledge-based and the empirical. Since morphological knowledge plays an essential role in any higherlevel understanding and processing of Arabic text, the book also features a part on the role of Arabic morphology in larger applications, i.e. Information Retrieval IR ; and Machine Translation MT.

Takara Shuzo Co., Japan ; , and then ligated to the SmaI fragment of the cI-spc cassette from pCISP303B#6 15 ; , resulting in plasmid pBRE2H4cI, containing the LPA. Preparation of a recipient strain containing the LPA. The Pr-neo cassette introduction plasmid was constructed with a substitutional insertion of an EcoRI fragment of Pr-neo from pBEST515C 14 ; between two NotI sites in pNEXT4 11 ; . The resulting plasmid, pNEXT4PN-2, was used to transform B. subtilis RM125, a strain 168 derivative that lacks a restriction modification system, generating BEST6225. The LPA carried by pBRE2H4cI was inserted into the leuB region in the BEST6225 genome as follows. Plasmid pBR322Cm 12 ; , which harbors a chloramphenicol resistance gene cat ; cassette in the EcoRI site of pBR322, was linearized at a unique PvuII site and then inserted into a unique BamHI site in the leuB gene of plasmid RSF2124.B.leuB 25 ; by using a T4 DNA polymerase-based blunting kit. The resulting plasmid, pBMAP103-322CA, was used to transform BEST6225, generating BEST6234, which has pBR322Cm inserted in the genomic leuB region Fig. 1C ; . BEST6234 was transformed with pBRE2H4cI and selected using spectinomycin. Transformants were assayed for chloramphenicol sensitivity by plate replication, and then a chloramphenicolsensitive strain, which resulted from the double-crossover recombination between pBR322Cm and pBRE2H4cI Fig. 1C ; , was selected. The obtained strain was designated 6234 cI and used as the recipient of the iturin A operon. Iturin A operon transfer. High-molecular-weight whole DNA of strain RB14 was prepared according to a method reported previously 11 ; . One hundred microliters of competent cell culture of 6234 cI was mixed with 10 l of RB14 chromosome. Following incubation at 37C for 30 min, 300 l of LB medium was added to the culture, which was incubated with gentle agitation at 37C for 3 h to allow the expression of neomycin resistance. The culture was then plated on LB plates containing neomycin and incubated at 30C overnight. Colonies that appeared on the plates were streaked on two LB plates, one containing spectinomycin and the other containing neomycin, for the screening of spectinomycin-sensitive colonies. Selected colonies were then picked up with a toothpick and inoculated in 25 l PCR solution 5 U of TaKaRa Ex Taq DNA polymerase [Takara Shuzo, Kyoto, Japan], 10 l of Ex Taq buffer, and 8 l of deoxynucleoside triphosphate solution [2.5 mM each] ; with the primers ITUP1-F 5 -AGCTTAGGGAACAATTGTCATCGGGGCTTC-3 , positioned from nucleotide 15353 to 15383 of the iturin A operon sequence [DDBJ EMBL GenBank accession no. AB050629] ; and ITUP2-R 5 -TCAGATAGGCCGCC ATATCGGAATGATTCG-3 , complementary sequence positioned from nucleotide 17326 to 17355 of AB050629 ; , which are able to detect a 2-kb region that includes the intergenic sequence between ituA and ituB. The colony PCR conditions were as follows: 96C for 5 min; 30 cycles of 96C for 30 s, 60C for 30 s, and 72C for 150 s. Introduction of sfp and degQ. The sfp-harboring E. coli plasmid pMMN6 26 ; was inserted into the genome of 6234 itu by Campbell-type insertion. In this transformation, genomic DNA of RM125 was simultaneously transferred to remove Pr-neo from the yvfC-yveP region for the following experiment. Thus, a chloramphenicol-resistant, neomycin-sensitive colony was selected and designated RM iS2. This strain harbors pMMN6 in the sfp0 region. We did not determine in which site the actual insertion in RM iS2 occurred. The degQYB8containing E. coli plasmid pUC19HP1NmrF 32 ; was transformed into the RM iS2 strain and selected for neomycin. Since pUC19HP1NmrF has three potential sites for Campbell-type insertion in the RM iS2 genome one is degQ0 and the others are ampicillin resistance genes in genomic pBR322 and pMMN6 ; , several transformants were selected and designated the RM iSd series. Quantitative analysis of iturin A, plipastatin, and surfactin. The culture 40 ml of no. 3S medium, 30C ; of the B. subtilis strain was acidified to pH 2.0 with 12 N HCl. Then, the precipitate formed was collected by centrifugation and extracted with methanol. Iturin A, plipastatin, and surfactin in the extracted solution were quantified by reversed-phase high-performance liquid chromatography HPLC ; using a two-eluent gradient as described previously 32, 33 ; . For the detailed composition analysis of the fatty moiety of the -amino acid of iturin A, the methanol extract was subjected to another reversed-phase HPLC using one eluent as described previously 8 and stadol.

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Not covered: Assisted reproductive technology ART ; procedures, such as: - embryo transplant, gamete intra-fallopian transfer GIFT ; and zygote intra-fallopian transfer ZIFT ; , sex selection, surrogacy, host uterus, gene therapy, cryopreservation, and pre-implantation genetic diagnosis Services and supplies related to ART procedures Cost of donor sperm and related costs Cost of donor egg and related costs Infertility services after voluntary sterilization or reversal of voluntary sterilization of either partner. All charges.

La Jolla, Calif. ; , and ligated into the EcoRI site of pUC18. The ligation mixture was used to transform E. coli DH5a. Approximately 400 ampicillin-resistant colonies were screened by colony hybridization, using end-labelled oligonucleotide as the probe. Two hybridizing colonies were chosen for further analysis. The plasmids from these two colonies both contained a 4.7-kb EcoRI fragment that hybridized with the oligonucleotide probe. One of these plasmids, pBH500, was chosen for all further work. Isolation of sigC. The Anabaena sp. strain PCC 7120 sigC gene was isolated by screening a cosmid library consisting of Anabaena sp. strain PCC 7120 DNA partially digested with Cpfl and cloned into the BglII site of cosmid vector pWB79 ; by colony hybridization, using as the probe a 740-bp AccI-EcoRI fragment of the Anabaena sp. strain PCC 7120 sigA gene 3 ; . This resulted in the isolation of a number of reactive clones, one of which, pCPF3, contained a 3.4-kb HincII fragment that hybridized with the probe. The 3.4-kb HincII fragment of pCPF3 was ligated into the HincII site of pUC19 to yield pBH700. pBH720, containing the insertion in the orientation opposite the orientation in pBH700, was generated by cutting pBH700 with HincHI, religating, and screening by restriction enzyme digestion. Disruption of sigB and sigC. Anabaena sp. strain DR1, in which the genomic sigB gene is interrupted by a spectinomycin-streptomycin resistance cassette, was constructed as described below. The 2-kb spectinomycin-streptomycin resistance cassette of pDW9 13 ; was excised with HincII and ligated into the end-repaired NheI site of pBH500 to yield pBH501. pBH502 was constructed by ligating the 6.7-kb EcoRI fragment of pBH501 into the EcoRI site of pBR322. The 6.7-kb EcoRI fragment from pBH502 was end repaired and ligated into the NruI site of pRL271 6 ; to yield pBH503. pBH503 was used to transform E. coli HB101 pRL528 ; 9 ; for conjugation into Anabaena sp. strain PCC 7120. For triparental matings we used standard procedures 9 ; . Similar methods were used to construct Anabaena sp. strain C9, in which the genomic sigC gene is interrupted by a spectinomycin-streptomycin resistance cassette. pBH701, in which the 2-kb spectinomycin-streptomycin resistance cassette replaces a 600-bp EcoRV internal fragment of sigC, was constructed by cutting pBH700 with EcoRV, isolating the large fragment, and ligating into it the spectinomycinstreptomycin resistance cassette from pDW9 cut with HincII. The 4.8-kb HincII fragment of pBH701 was then ligated into the NruI site of pRL271 to yield pBH702, which was used to transform E. coli MC1061 pRL528 ; for conjugation into Anabaena sp. strain PCC 7120. Exconjugants were selected by plating the mating mixture onto BG11 plates containing 2 Fg of spectinomycin per ml and 2 , g of streptomycin per ml. Single recombinant colonies in which all of pBH503 or all of pBH702 had integrated into the chromosomal copy of sigB or sigC, respectively, appeared within 10 to 14 days. Double recombinants were selected as described previously 6 ; on BGll plates containing 5% sucrose, 2 , g of spectinomycin per ml, and 2 , ug of streptomycin per ml. Presumptive single and double recombinants were screened by Southern hybridization to confirm the expected restriction pattern that was indicative of two copies of sigB or sigC one interrupted ; and plasmid sequences in the single recombinant or one copy of the interrupted sigB or sigC gene in the double recombinants data not shown ; . Nucleotide sequence accession numbers. The DNA sequences reported in this paper are available from GenBank under accession numbers M95760 sigB ; and M95759 sigC and stanozolol.

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Defined their rat unit as the total dose of PMSG which will produce a 5-fold increase in ovarian weight. In 1938 an international standard for PMSG was established. One International Unit was defined as 0.25 mg of the standard preparation Burn, 1950 ; . Soon afterwards commercial preparations of PMSG appeared on the market. Schering Corporation in the USA marketed Anteron, and Organon and Roussell in Europe marketed `Gestyl' and `Gonadotrophine Serique'. Both preparations were reimbursed by `Social Security' in France. Clinical trials in women demonstrated an ovarian response to these gonadotrophins Fevold et al., 1931; Fevold, 1937; Hamblen, 1940 ; , but attempts to induce ovulation produced inconsistent results. In 1939, Hamblen showed that `cyclic administration of PMSG during the follicular phase of the cycle, in amounts judged to be adequate, failed to result in progestational bleeding or progestational changes in the endometrium as judged by endometrial biopsy studies ; or in pregnancy' Hamblen, 1935, Hamblen, 1939 ; . not withdrawn until July 6, 1972 see the Federal Register of July 6, 1972: 37 FR 13284 ; . As late as 1962, Folistiman VEB Arneimittelwerk Dresden ; , a highly purified, standardized FSH preparation from pig pituitaries, was introduced on the East German market. These preparations were used in the hope that perhaps ovulation and consequent pregnancy could be evoked within the first few months of treatment, before the immune response and its consequences had fully developed. A number of pregnancies were indeed reported Vesell, 1938; Rydberg and Madsen, 1949; Rydberg and Ostergaard, 1939; Daume, 1970; Groot-Wassink and Blawert, 1973 ; . Daume compared the results of ovulation induction by sheep gonadotrophin extract with those obtained using urinary hMG. The pregnancy rate per treatment cycle was 11.5 and 12.7% for the animal and human preparations respectively. Groot-Wassink and Blawert 1973 ; compared the results pregnancy rate ; of an animal and a human FSH preparation, and found that gonadotrophins derived from animal sources gave significantly better results. They explained this difference on the basis that poor results obtained with preparations derived from human sources were due to an excess of LH: the FSH: LH ratio was 11: 1 in the human preparation, and 70: 1 in the animal preparation. Groot-Wassink and Blawert 1973 ; were the first to claim that excessive LH could have adverse effects on reproductive performance. PMSG eventually had to be withdrawn from the market because of the potential dangers as a consequence of provoking antibody formation. However, animal gonadotrophins under the trade name Folistiman VEB Arzneimittelwerk, Dresden ; were still available in some East European countries until 1998. Recognizing the fact that animal gonadotrophins might produce antibodies in humans which could neutralize not only the preparation applied, but also the endogenous gonadotrophins, scientific and technological efforts were focused on extracting and purifying gonadotrophins from human sources. During the summer of 1953, Dr Rudi Borth and myself, with the help of Prof. H. de Watteville invited a number of scientists to Geneva in order to exchange information and to coordinate research on gonadotrophins. Egon Diczfalusy, Jim Brown, John Loraine and others were amongst those invited. The `G Club' was founded during this meeting, and the basic and clinical goals of gonadotrophic research defined. These included development of specific assay procedures, as well as bioassay standards and purification methods required to obtain gonadotrophic preparations suitable for therapeutic purposes.

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Prostaglandins work by changing water and electrolyte absorption in the intestines. It also is believed that the laxative effect may be due to the motor effect of prostaglandins, but this is not yet clear. Researchers found that misoprostol, marketed as Cytotec, increased the weight and frequency of stools and shortened colonic transit time in patients with severe chronic constipation.32 Cisapride, an agent that stimulates the upper gastrointestinal tract, is useful in patients with spinal cord injury or Parkinson's disease.27 Colchicine, used to treat gouty arthritis, is useful in treatment of chronic constipation.33 No studies on the effectiveness of these drugs for opioidinduced constipation have been reported and stelazine.
From the 1Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts; the 2Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts; and the 3Departments of Nutrition and Epidemiology, Harvard School of Public Health, Boston, Massachusetts. Address correspondence and reprint requests to Eric N. Taylor, MD, Channing Laboratory, Brigham and Women's Hospital, 181 Longwood Ave., Boston, MA 02115. E-mail: entaylor partners . Received for publication 2 December 2005 and accepted in revised form 20 January 2006. Abbreviations: ALLHAT, Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial; ARIC, Atherosclerosis Risk in Communities; ASCOT, Anglo-Scandinavian Cardiac Outcomes Trial; FFQ, food frequency questionnaire; NHS, Nurses' Health Study; HPFS, Health Professionals Follow-up Study. A table elsewhere in this issue shows conventional and Systeme International SI ; units and conversion ` factors for many substances. DOI: 10.2337 dc05-2366 2006 by the American Diabetes Association.

Patient Age yr ; 1 2 Hgb at Presentation gm dL ; 7.6 7.9 11.2 Location of Dieulafoy's Lesion Esophagus Esophagus Cardia of Stomach Body of Stomach Cardia of Stomach Fundus of Stomach Body of Stomach Body of Stomach Body of Stomach Body of Stomach Body of Stomach Body of Stomach Duodenum Fundus Fundus Endoscopic Treatment Epinephrine Epi + Heater Probe Epinephrine Epinephrine Epinephrine Epi + Heater Probe Epi + Heater Probe Epi + Heater Probe Epi + Heater Probe Heater Probe Epi + Heater Probe Epi + Heater Probe Epinephrine Epi + Endoclip Epi + Endoclip and suboxone.
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Significance of the changes vs month 0 in each group: a1, P-0.05; a2, P-0.02. Significance of the difference between the two groups at each month: b2, P-0.02. S, serum; 25-OH D, 25-OH vitamin D; P, plasma; DCa, dialysate calcium; Nb pt, patient number and subutex.
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Crystal arthropathies Chondrocalcinosis due to dicalcium phosphate crystals Code first underlying disease 275.4 ; 712.2x Chondrocalcinosis due to pyrophosphate crystals Code first underlying disease 275.4 ; 712.3x Chondrocalcinosis, unspecified Code first underlying disease 275.4 ; 712.8x Other specified crystal arthropathies 712.9x Unspecified crystal arthropathy 714.x 714.0 714.1 Rheumatoid arthritis and other inflammatory polyarthropathies Rheumatoid arthritis Felty's syndrome Other rheumatoid arthritis with visceral or systemic involvement Juvenile chronic polyarthritis Polyarticular juvenile rheumatoid arthritis, chronic or unspecified Polyarticular juvenile rheumatoid arthritis, acute Pauciarticular juvenile rheumatoid arthritis Monoarticular juvenile rheumatoid arthritis Chronic postrheumatic arthropathy Other specified inflammatory polyarthropathies Rheumatoid lung Other Unspecified inflammatory polyarthropathy Osteoarthrosis and allied disorders Osteoarthrosis, generalized Osteoarthrosis, localized, primary Osteoarthrosis, localized, secondary Osteoarthrosis, localized, not specified whether primary or secondary Osteoarthrosis involving, or with mention of more than one site, but not specified as generalized Osteoarthrosis, unspecified whether generalized or localized Other and unspecified arthropathies Kaschin-Beck disease Traumatic arthropathy Allergic arthritis Climacteric arthritis Transient arthropathy Unspecified polyarthropathy or polyarthritis Unspecified monoarthritis Other specified arthropathy Arthropathy, unspecified Other derangement of joint Articular cartilage disorder Loose body in joint Pathological dislocation Recurrent dislocation of joint Contracture of joint Ankylosis of joint Unspecified intrapelvic protrusion of acetabulum Developmental dislocation of joint Other joint derangement, not elsewhere
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H NMR spectra were recorded on a Varian spectrometer Varian Analytical Instruments, Sunnyvale, CA ; operating at a frequency of 300 MHz. The proton chemical shift values were listed in parts per million relative to tetramethylsilane. Fast atom bombardment FAB ; mass spectra were recorded on a VG 70SEQ instrument. Both instruments are housed at the Department of Medicinal Chemistry, University of Washington Seattle, WA ; . Liquid chromatographyatmospheric pressure-chemical ionization-mass spectra were recorded with a VG Platform single quadrupole mass spectrometer at the National Center for Toxicological Research Jefferson, AR and sudafed. GRANTS This work was supported by National Heart, Lung, and Blood Institute Grants HL-076361 to D. J. Milan ; , HL-071632 to P. T. Ellinor ; , and HL-075431 to C. A. MacRae ; . D. J. Milan was also supported by a grant from the Cardiovascular Research Foundation and spiriva.
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